Green Synthesis of Silver Nanoparticles: A Comprehensive Review For Sustainable Nanotechnology Applications
DOI:
https://doi.org/10.54392/Keywords:
Ag Nanoparticles, Green Synthesis, Sensors, Environment, EnergyAbstract
An eco-friendly and biocompatible alternative to the conventional chemical and physical synthesis methods is the green synthesis of silver nanoparticles (AgNPs). The synthesis of AgNP through plant, microbial, algal and enzyme is critically examined in this comprehensive overview. While nucleation and growth kinetics are governed by reaction parameters such as pH, temperature and extract concentration, information derived from spectroscopy, electron microscopy and computational modelling identifies phenolic, amino and protein functionalities to dominate reduction and stabilization. Green AgNPs have a wide range of functional variety in biomedical, environmental and catalytic applications. They have strong antibacterial, anticancer, photocatalytic and sensing properties. Although there are still challenges with reproducibility, mass production and long-term durability, comparative studies show advantages in energy efficiency, safety and environmental compatibility. Predictive control over particle qualities may be possible through the combination of experimental optimization with artificial intelligence (AI) and machine learning (ML). To ensure eco-friendly and industrially scalable applications, the emerging fields include hybrid synthesis techniques, life-cycle-oriented risk assessment and regulatory coordination. The integration of AI-based design and green chemistry is a ground-breaking approach to sustainable nanotechnology.
References
R.R. Vidyasagar, S.K. Patel, M. Singh, S.K. Singh, Green synthesis of silver nanoparticles: methods, biological applications, delivery and toxicity, Materials Advances 4 (7) (2023) 1831–1849. https://doi.org/10.1039/D2MA01105K
R. Vishwanath, B. Negi, Conventional and green methods of synthesis of silver nanoparticles and their antimicrobial properties. Current Research in Green and Sustainable Chemistry, 4, (2021) 100205. https://doi.org/10.1016/j.crgsc.2021.100205
D. Kumar, S. Kumar, A.D. Gholap, R. Kumari, R. Tanwar, V. Kumar, M. Khalid, M.A. Faiyazuddin, comprehensive review on the biomass-mediated synthesis of silver nanoparticles: Opportunities and challenges. Journal of Environmental Chemical Engineering, 13(1), (2024) 115133. https://doi.org/10.1016/j.jece.2024.115133
M.R. Khan, M.A. Urmi, C. Kamaraj, G. Malafaia, C. Ragavendran, M.M. Rahman, Green synthesis of silver nanoparticles with its bioactivity, toxicity and environmental applications: A comprehensive literature review. Environmental Nanotechnology, Monitoring & Management, 20, (2023) 100872. https://doi.org/10.1016/j.enmm.2023.100872
F. Eker, E. Akdaşçi, H. Duman, M. Bechelany, S. Karav, Green Synthesis of Silver Nanoparticles Using Plant Extracts: A Comprehensive Review of Physicochemical Properties and Multifunctional Applications. International Journal of Molecular Sciences, 26(13), (2025) 6222. https://doi.org/10.3390/ijms26136222
S. Shahzadi, Z. Fatima, M.N. Zafar, F.N. Zafar, M.R.S.A. Janjua, A review on green synthesis of silver nanoparticles (SNPs) using plant extracts: a multifaceted approach in photocatalysis, environmental remediation, and biomedicine. RSC Advances 15(5), (2025) 3858–3903. https://doi.org/10.1039/D4RA07519F
S. Parmar, H. Kaur, J. Singh, A.S. Matharu, S. Ramakrishna, M. Bechelany, Recent advances in green synthesis of Ag NPs for extenuating antimicrobial resistance. Nanomaterials, 12(7), (2022) 1115. https://doi.org/10.3390/nano12071115
S. Kaabipour, S. Hemmati, A review on the green and sustainable synthesis of silver nanoparticles and one-dimensional silver nanostructures. Beilstein Journal of Nanotechnology, 12(1), (2021) 102-136. https://doi.org/10.3762/bjnano.12.9
M. Moond, S. Singh, S. Sangwan, R. Devi, R. Beniwal, (2022). Green synthesis and applications of silver nanoparticles: a systematic review. AATCC Journal of Research, 9(6), 272-285. https://doi.org/10.1177/24723444221119847
D. Gupta, A. Boora, A. Thakur, T.K. Gupta, Green and sustainable synthesis of nanomaterials: recent advancements and limitations. Environmental Research, 231, (2023) 116316. https://doi.org/10.1016/j.envres.2023.116316
M. Fahim, A. Shahzaib, N. Nishat, A. Jahan, T.A. Bhat, A. Inam, Green synthesis of silver nanoparticles: A comprehensive review of methods, influencing factors, and applications. JCIS Open, 16, (2024) 100125. https://doi.org/10.1016/j.jciso.2024.100125
A.I. Osman, Y. Zhang, M. Farghali, A.K. Rashwan, A.S. Eltaweil, E.M. Abd El-Monaem, M.M. Badr, I. Ihara, D.W. Rooney, P.S. Yap, Synthesis of green nanoparticles for energy, biomedical, environmental, agricultural, and food applications: A review. Environmental Chemistry Letters, 22(2), (2024) 841-887. https://doi.org/10.1007/s10311-023-01682-3
A. Dhaka, S.C. Mali, S. Sharma, R. Trivedi, A review on biological synthesis of silver nanoparticles and their potential applications. Results in Chemistry, 6, (2023) 101108. https://doi.org/10.1016/j.rechem.2023.101108
S.J. Nadaf, N.R. Jadhav, H.S. Naikwadi, P.L. Savekar, I.D. Sapkal, M.M. Kambli, I.A. Desai, Green synthesis of gold and silver nanoparticles: Updates on research, patents, and future prospects. OpenNano, 8, (2022) 100076. https://doi.org/10.1016/j.onano.2022.100076
T.L. Pham, V.D. Doan, Q. Le Dang, T.A. Nguyen, T.L.H. Nguyen, T.D.T. Tran, T.P.L. Nguyen, T.K.A. Vo, T.H. Nguyen, D.L. Tran, Stable biogenic silver nanoparticles from Syzygium nervosum bud extract for enhanced catalytic, antibacterial and antifungal properties. RSC advances, 13(30), (2023) 20994-21007. https://doi.org/10.1039/D3RA02754F
A. Wirwis, Z. Sadowski, Green synthesis of silver nanoparticles: optimizing green tea leaf extraction for enhanced physicochemical properties. ACS omega, 8(33), (2023) 30532-30549. https://doi.org/10.1021/acsomega.3c03775
R. Kaur, P. Avti, V. Kumar, R. Kumar, Effect of various synthesis parameters on the stability of size controlled green synthesis of silver nanoparticles. Nano Express, 2(2), (2021) 020005. https://doi.org/10.1088/2632-959X/abf42a
G. Habibullah, J. Viktorova, P. Ulbrich, T. Ruml, Effect of the physicochemical changes in the antimicrobial durability of green synthesized silver nanoparticles during their long-term storage. RSC advances, 12(47), (2022) 30386-30403. https://doi.org/10.1039/D2RA04667A
C. Vanlalveni, S. Lallianrawna, A. Biswas, M. Selvaraj, B. Changmai, S.L. Rokhum, Green synthesis of silver nanoparticles using plant extracts and their antimicrobial activities: A review of recent literature. RSC advances, 11(5), (2021) 2804-2837. https://doi.org/10.1039/D0RA09941D
S.L. Ramírez-Rosas, E. Delgado-Alvarado, L.O. Sanchez-Vargas, A.L. Herrera-May, M.G. Peña-Juarez, J.A. Gonzalez-Calderon, Green route to produce silver nanoparticles using the bioactive flavonoid quercetin as a reducing agent and food anti-caking agents as stabilizers. Nanomaterials, 12(19), (2022) 3545. https://doi.org/10.3390/nano12193545
P.K. Tyagi, S. Tyagi, D. Gola, A. Arya, S.A. Ayatollahi, M.M. Alshehri, J. Sharifi-Rad, Ascorbic acid and polyphenols mediated green synthesis of silver nanoparticles from Tagetes erecta L. aqueous leaf extract and studied their antioxidant properties. Journal of Nanomaterials, 2021(1), (2021) 6515419. https://doi.org/10.1155/2021/6515419
C. Pattnaik, R. Mishra, A.K. Sahu, L.N. Sahoo, N.K. Sahoo, S.K. Tripathy, S. Sahoo, Green synthesis of glucose-capped stable silver nanoparticles: a cost-effective sensor for the selective detection of Hg 2+ ions in aqueous solutions. Sensors & Diagnostics, 2(3), (2023) 647-656. https://doi.org/10.1039/D3SD00019B
E. Mirda, R. Idroes, K. Khairan, T.E. Tallei, M. Ramli, N. Earlia, A. Maulana, G. Mauer Idroes, M. Muslem, Z. Jalil, Synthesis of chitosan-silver nanoparticle composite spheres and their antimicrobial activities. Polymers, 13(22), (2021) 3990. https://doi.org/10.3390/polym13223990
D.J. Boehmler, Z.J. O’Dell, C. Chung, K.R. Riley, Bovine serum albumin enhances silver nanoparticle dissolution kinetics in a size-and concentration-dependent manner. Langmuir, 36(4), (2020) 1053-1061. https://doi.org/10.1021/acs.langmuir.9b03251
N.M. El-Deeb, M.A. Abo-Eleneen, L.A. Al-Madboly, M.M. Sharaf, S.S. Othman, O.M. Ibrahim, M.S. Mubarak, Biogenically synthesized polysaccharides-capped silver nanoparticles: immunomodulatory and antibacterial potentialities against resistant Pseudomonas aeruginosa. Frontiers in Bioengineering and Biotechnology, 8, (2020) 643. https://doi.org/10.3389/fbioe.2020.00643
N.M. El-Deeb, M.A. Abo-Eleneen, L.A. Al-Madboly, M.M. Sharaf, S.S. Othman, O.M. Ibrahim, M.S. Mubarak, Biogenically synthesized polysaccharides-capped silver nanoparticles: immunomodulatory and antibacterial potentialities against resistant Pseudomonas aeruginosa. Frontiers in Bioengineering and Biotechnology, 8, (2020) 643. https://doi.org/10.1186/s43088-019-0031-y
N. Cardullo, V. Muccilli, C. Tringali, Laccase-mediated synthesis of bioactive natural products and their analogues. RSC Chemical Biology, 3(6), (2022) 614-647. https://doi.org/10.1039/D1CB00259G
N. Liaqat, N. Jahan, T. Anwar, H. Qureshi, Green synthesized silver nanoparticles: Optimization, characterization, antimicrobial activity, and cytotoxicity study by hemolysis assay. Frontiers in chemistry, 10, (2022) 952006. https://doi.org/10.3389/fchem.2022.952006
M.M. El-Sheekh, M.A. Deyab, N.I. Hassan, S.E. Abu Ahmed, (2022). Green biosynthesis of silver nanoparticles using sodium alginate extracted from Sargassum latifolium and their antibacterial activity. Rendiconti Lincei. Scienze Fisiche e Naturali, 33(4), 867-878. https://doi.org/10.1007/s12210-022-01102-8
A. Khan, T. Younis, M. Anas, M. Ali, Z.K. Shinwari, A.T. Khalil, K. Shahzad Munawar, H.E.A. Mohamed, K. Hkiri, M. Maaza, M.F. Seleiman Khan, N. Withania, coagulans-mediated green synthesis of silver nanoparticles: characterization and assessment of their phytochemical, antioxidant, toxicity, and antimicrobial activities. BMC Plant Biology, 25(1), (2025) 574. https://doi.org/10.1186/s12870-025-06533-7
A.M. Abdel-Aty, A.Z. Barakat, R.I. Bassuiny, S.A. Mohamed, Statistical optimization, characterization, antioxidant and antibacterial properties of silver nanoparticle biosynthesized by saw palmetto seed phenolic extract. Scientific Reports, 13(1), (2023) 15605. https://doi.org/10.1038/s41598-023-42675-0
M.J. Islam, N. Khatun, R.H. Bhuiyan, S. Sultana, M.A.A. Shaikh, M.N.A. Bitu, F. Chowdhury, S. Islam, Psidium guajava leaf extract mediated green synthesis of silver nanoparticles and its application in antibacterial coatings. RSC advances, 13(28), (2023) 19164-19172. https://doi.org/10.1039/D3RA03381C
P. Gupta, N. Rai, A. Verma, D. Saikia, S.P. Singh, R. Kumar, S.K. Singh, D. Kumar, V. Gautam, Green-Based Approach to Synthesize Silver Nanoparticles Using the Fungal Endophyte Penicillium oxalicum and Their Antimicrobial, Antioxidant, and In Vitro Anticancer Potential. ACS omega, 8(33), (2023). 30788. https://doi.org/10.1021/acsomega.3c05396
M.A. Basheer, K. Abutaleb, N.N. Abed, A.A. Mekawey, Mycosynthesis of silver nanoparticles using marine fungi and their antimicrobial activity against pathogenic microorganisms. Journal of Genetic Engineering and Biotechnology, 21(1), (2023) 127. https://doi.org/10.1186/s43141-023-00572-z
A. Dede, M.T. Aytekin-Aydin, K. Güven, Green Biosynthesis of Silver Nanoparticles from Olive and Walnut-Related Bacteria, Synthesis, Characterization, and Antimicrobial Activity. Indian Journal of Microbiology, 63(4), (2023) 658-667. https://doi.org/10.1007/s12088-023-01127-z
S.S. Salem, O.M. Ali, A.M. Reyad, K.A. Abd-Elsalam, A.H. Hashem, Pseudomonas indica-mediated silver nanoparticles: Antifungal and
antioxidant biogenic tool for suppressing mucormycosis fungi. Journal of Fungi, 8(2), (2022) 126. https://doi.org/10.3390/jof8020126
R. Algotiml, A. Gab-Alla, R. Seoudi, H.H. Abulreesh, M.Z. El-Readi, K. Elbanna, Anticancer and antimicrobial activity of biosynthesized Red Sea marine algal silver nanoparticles. Scientific Reports, 12(1), (2022) 2421. https://doi.org/10.1038/s41598-022-06412-3
C.K. Somasundaram, R. Atchudan, T. N. J. I. Edison, S. Perumal, R. Vinodh, A.K. Sundramoorthy, R.S. Babu, M. Alagan, Y.R. Lee, Sustainable synthesis of silver nanoparticles using marine algae for catalytic degradation of methylene blue. Catalysts, 11(11), (2021) 1377. https://doi.org/10.3390/catal11111377
T.A. Devi, R.M. Sivaraman, S.S. Thavamani, T.P. Amaladhas, M.S. AlSalhi, S. Devanesan, M.M. Kannan, Green synthesis of plasmonic nanoparticles using Sargassum ilicifolium and application in photocatalytic degradation of cationic dyes. Environmental Research, 208, (2022) 112642. https://doi.org/10.1016/j.envres.2021.112642
M. Saba, S. Farooq, A.H. Alessa, K.I. Bektas, A.O. Belduz, A.Z. Khan, A.A. Shah, M. Badshah, S. Khan, Green synthesis of silver nanoparticles using Keratinase from Pseudomonas aeruginosa-C1M, characterization and applications as novel multifunctional biocatalyst. BMC biotechnology, 25(1), (2025) 27. https://doi.org/10.1186/s12896-025-00959-5
E. Hermosilla, M. Díaz, J. Vera, M.J. Contreras, K. Leal, R. Salazar, L. Barrientos, G. Tortella, O. Rubilar, Synthesis of antimicrobial chitosan-silver nanoparticles mediated by reusable chitosan fungal beads. International journal of molecular sciences, 24(3), (2023) 2318. https://doi.org/10.3390/ijms24032318
Ritu, K.K. Verma, A. Das, P. Chandra, Phytochemical-based synthesis of silver nanoparticle: mechanism and potential applications. BioNanoScience, 13(3), (2023) 1359-1380. https://doi.org/10.1007/s12668-023-01125-x
T. Ongtanasup, P. Kamdenlek, C. Manaspon, K. Eawsakul, Green-synthesized silver nanoparticles from Zingiber officinale extract: antioxidant potential, biocompatibility, anti-LOX properties, and in silico analysis. BMC Complementary Medicine and Therapies, 24(1), (2024) 84. https://doi.org/10.1186/s12906-024-04381-w
M. Kassem Agha, B. Maatouk, R. Mhanna, M.H. El-Dakdouki, Catalytic degradation efficacy of silver nanoparticles fabricated using Actinidia deliciosa peel extract. Journal of Nanomaterials, 2024(1), (2024) 8813109. https://doi.org/10.1155/2024/8813109
I. Kumar, C. Gangwar, B. Yaseen, P.K. Pandey, S.K. Mishra, R.M. Naik, Kinetic and mechanistic studies of the formation of silver nanoparticles by nicotinamide as a reducing agent. ACS omega, 7(16), (2022) 13778-13788. https://doi.org/10.1021/acsomega.2c00046
B. Barik, B.S. Satapathy, G. Pattnaik, D.V. Bhavrao, K.P. Shetty, Sustainable synthesis of silver nanoparticles from Azadirachta indica: antimicrobial, antioxidant and in silico analysis for periodontal treatment. Frontiers in Chemistry, 12, (2024) 1489253. https://doi.org/10.3389/fchem.2024.1489253
A. Corciovă, C. Mircea, A.F. Burlec, A. Fifere, I.T. Moleavin, A. Sarghi, C. Tuchiluș, B. Ivănescu, I. Macovei, Green synthesis and characterization of silver nanoparticles using a Lythrum salicaria extract and in vitro exploration of their biological activities. Life, 12(10), (2022) 1643. https://doi.org/10.3390/life12101643
M. Asif, R. Yasmin, R. Asif, A. Ambreen, M. Mustafa, S. Umbreen, Green synthesis of silver nanoparticles (AgNPs), structural characterization, and their antibacterial potential. Dose-response, 20(2), (2022) 15593258221088709. https://doi.org/10.1177/15593258221088709
S. Pasieczna-Patkowska, M. Cichy, J. Flieger, Application of Fourier transform infrared (FTIR) spectroscopy in characterization of green synthesized nanoparticles. Molecules, 30(3), (2025) 684. https://doi.org/10.3390/molecules30030684
P. Singh, I. Mijakovic, Strong antimicrobial activity of silver nanoparticles obtained by the green synthesis in Viridibacillus sp. extracts. Frontiers in microbiology, 13, (2022) 820048. https://doi.org/10.3389/fmicb.2022.820048
N.K. Sharma, J. Vishwakarma, S. Rai, T.S. Alomar, N. AlMasoud, A. Bhattarai, Green route synthesis and characterization techniques of silver nanoparticles and their biological adeptness. ACS omega, 7(31), (2022) 27004-27020. https://doi.org/10.1021/acsomega.2c01400
S. Singaravelu, F. Motsoene, H. Abrahamse, S.S. Dhilip Kumar, Green-synthesized metal nanoparticles: a promising approach for accelerated wound healing. Frontiers in bioengineering and biotechnology, 13, (2025) 1637589. https://doi.org/10.3389/fbioe.2025.1637589
F. Nosrati, B. Fakheri, H. Ghaznavi, N. Mahdinezhad, R. Sheervalilou, B. Fazeli-Nasab, Green synthesis of silver nanoparticles from plant Astragalus fasciculifolius Bioss and evaluating cytotoxic effects on MCF7 human breast cancer cells. Scientific Reports, 15(1), (2025) 25474. https://doi.org/10.1038/s41598-025-05224-5
D.M. Radulescu, V.A. Surdu, A. Ficai, D. Ficai, A.M. Grumezescu, E. Andronescu, Green synthesis of metal and metal oxide nanoparticles: a review of the principles and biomedical applications. International Journal of Molecular Sciences, 24(20), (2023) 15397. https://doi.org/10.3390/ijms242015397
T.T.T. Nguyen, N.T.K. Tran, T.Q. Le, T.T.A. Nguyen, L.T.M. Nguyen, T. Van Tran, Passion fruit peel pectin/chitosan based antibacterial films incorporated with biosynthesized silver nanoparticles for wound healing application. Alexandria Engineering Journal, 69, (2023) 419-430. https://doi.org/10.1016/j.aej.2023.01.066
W. Chu, P. Wang, Z. Ma, L. Peng, C. Guo, Y. Fu, L. Ding, Lupeol-loaded chitosan-Ag+ nanoparticle/sericin hydrogel accelerates wound healing and effectively inhibits bacterial infection. International Journal of Biological Macromolecules, 243, (2023) 125310. https://doi.org/10.1016/j.ijbiomac.2023.125310
Sharma, S., Bose, A., Biswas, S., Sen, S., & Roy, I. (2025). Cyperus rotundus mediated green synthesis of silver nanoparticles for antibacterial wound dressing applications. Scientific Reports, 15(1), 18394. https://doi.org/10.1038/s41598-025-03555-x
J. Nandhini, E. Karthikeyan, E.E. Rani, V.S. Karthikha, D.S. Sanjana, H. Jeevitha, S. Rajeshkumar, V. Venugopal, A. Priyadharshan, Advancing engineered approaches for sustainable wound regeneration and repair: harnessing the potential of green synthesized silver nanoparticles. Engineered Regeneration, 5(3), (2024) 306-325. https://doi.org/10.1016/j.engreg.2024.06.004
M. Tharani, S. Rajeshkumar, K.A. Al-Ghanim, M. Nicoletti, N. Sachivkina, M. Govindarajan, Terminalia chebula-assisted silver nanoparticles: biological potential, synthesis, characterization, and ecotoxicity. Biomedicines, 11(5), (2023) 1472. https://doi.org/10.3390/biomedicines11051472
P. Kumar, J. Dixit, A.K. Singh, V.D. Rajput, P. Verma, K.N. Tiwari, S.K. Mishra, T. Minkina, Mandzhieva, S. (2022). Efficient catalytic degradation of selected toxic dyes by green biosynthesized silver nanoparticles using aqueous leaf extract of Cestrum nocturnum L. Nanomaterials, 12(21), 3851. https://doi.org/10.3390/nano12213851
S. Khan, T. Noor, N. Iqbal, L. Yaqoob, Photocatalytic dye degradation from textile wastewater: a review. ACS omega, 9(20), (2024) 21751-21767. https://doi.org/10.1021/acsomega.4c00887
M. Sarkar, S. Denrah, M. Das, M. Das, Statistical optimization of bio-mediated silver nanoparticles synthesis for use in catalytic degradation of some azo dyes. Chemical Physics Impact, 3, (2021) 100053. https://doi.org/10.1016/j.chphi.2021.100053
K.M. Aboelghait, W.E. Abdallah, I. Abdelfattah, A.M. El-Shamy, Green synthesis of silver nanoparticles by waste of Murcott Mandarin peel as a sustainable approach for efficient heavy metal removal from metal industrial wastewater. Separation and Purification Technology, 347, (2024) 127609. https://doi.org/10.1016/j.seppur.2024.127609
S.B.N. Krishna, A.G. Sheik, K. Pillay, M.A. Hamza, M.Y.M. Elamir, S. Selim, Nanotechnology in action: silver nanoparticles for improved eco-friendly remediation. PeerJ, 12, (2024) e18191. https://doi.org/10.7717/peerj.18191
N. Bashir, M. Afzaal, A.L. Khan, R. Nawaz, A. Irfan, K.S. Almaary, F. Dabiellil, M. Bourhia, Z. Ahmed, Green-synthesized silver nanoparticle-enhanced nanofiltration mixed matrix membranes for high-performance water purification. Scientific Reports, 15(1), (2025) 1001. https://doi.org/10.1038/s41598-024-83801-w
M. Dahooe Balooch, A. Naeimi, P. Iranmanesh, S. Saeednia, A Green Silver Nanoparticles using Pistachio Wastes for Dyes Degradation Exposing Visible Light and Reduction of 4‐Nitrophenol. ChemistrySelect, 8(29), (2023) e202300652. https://doi.org/10.1002/slct.202300652
N.H. Ibrahim, G.M. Taha, N.S.A. Hagaggi, M.A. Moghazy, Green synthesis of silver nanoparticles and its environmental sensor ability to some heavy metals. BMC chemistry, 18(1), (2024) 7. https://doi.org/10.1186/s13065-023-01105-y
P. Borpujari, S. Khan, J. Dutta, D. Borah, Biochar-metal nanocomposites for azo dye removal: advancements, mechanisms, and future prospects. Discover Applied Sciences, 7(9), (2025) 1023. https://doi.org/10.1007/s42452-025-07706-7
M. Mavaei, A. Chahardoli, Y. Shokoohinia, A. Khoshroo, A. Fattahi, One-step synthesized silver nanoparticles using isoimperatorin: evaluation of photocatalytic, and electrochemical activities. Scientific reports, 10(1), (2020) 1762. https://doi.org/10.1038/s41598-020-58697-x
P. Prema, V. Veeramanikandan, K. Rameshkumar, M.K. Gatasheh, A.A. Hatamleh, R. Balasubramani, P. Balaji, Statistical optimization of silver nanoparticle synthesis by green tea extract and its efficacy on colorimetric detection of mercury from industrial waste water. Environmental Research, 204, (2022) 111915. https://doi.org/10.1016/j.envres.2021.111915
K. Chand, D. Cao, D.E. Fouad, A.H. Shah, A.Q. Dayo, K. Zhu, L M.N. akhan, G. Mehdi, S. Dong, Green synthesis, characterization and photocatalytic application of silver nanoparticles synthesized by various plant extracts. Arabian Journal of Chemistry, 13(11), (2020) 8248-8261. https://doi.org/10.1016/j.arabjc.2020.01.009
A. Kumar, M.R.S. Savanur, S.K. Singh, J. Singh, S. Bhattacharya, K. Asnani, B. Kumar, M. Kumar, S. Kumar, Recent advances in silver nanoparticles‐catalyzed reactions. ChemistrySelect, 10(5), (2025) e202403655. https://doi.org/10.1002/slct.202403655
K.T. Khac, H.H. Phu, H.T. Thi, V.D. Thuy, H. Do Thi, Biosynthesis of silver nanoparticles using tea leaf extract (Camellia sinensis) for photocatalyst and antibacterial effect. Heliyon, 9(10), (2023). https://doi.org/10.1016/j.heliyon.2023.e20707
V.N. Anjana, M. Joseph, S. Francis, A. Joseph, E.P. Koshy, B. Mathew, Microwave assisted green synthesis of silver nanoparticles for optical, catalytic, biological and electrochemical applications. Artificial cells, nanomedicine, and biotechnology, 49(1), (2021) 438-449. https://doi.org/10.1080/21691401.2021.1925678
Z. Othman, A. Sinopoli, H.R. Mackey, K.A. Mahmoud, Efficient Photocatalytic Degradation of Organic Dyes by AgNPs/TiO2/Ti3C2Tx MXene Composites under UV and Solar Light. ACS Omega, 6(49), (2021) 33325–33338. https://doi.org/10.1021/acsomega.1c03189
M. Salve, A. Mandal, K. Amreen, P.K. Pattnaik, S. Goel, Greenly synthesized silver nanoparticles for supercapacitor and electrochemical sensing applications in a 3D printed microfluidic platform. Microchemical Journal, 157, (2020) 104973. https://doi.org/10.1016/j.microc.2020.104973
L.A. Faustino, L.D. de Angelis, E.C. de Melo, G. Farias, E.C. dos Santos, C.R. Miranda, A.G. Buzanich, R.M. Torresi, P.F. de Oliveira, S.I.C. de Torresi, Urea synthesis by Plasmon-Assisted N2 and CO2 co-electrolysis onto heterojunctions decorated with silver nanoparticles. Chemical Engineering Journal, 513, (2025) 163072. https://doi.org/10.1016/j.cej.2025.163072
T.N.J.I. Edison, R. Atchudan, N. Karthik, J. Balaji, D. Xiong, Y.R. Lee, Catalytic degradation of organic dyes using green synthesized N-doped carbon supported silver nanoparticles. Fuel, 280, (2020) 118682. https://doi.org/10.1016/j.fuel.2020.118682
F. Beck, M. Loessl, A.J. Baeumner, Signaling strategies of silver nanoparticles in optical and electrochemical biosensors: considering their potential for the point-of-care. Microchimica Acta, 190(3), (2023) 91. https://doi.org/10.1007/s00604-023-05666-6
N. Srikhao, A. Ounkaew, P. Kasemsiri, S. Theerakulpisut, M. Okhawilai, S. Hiziroglu, Green synthesis of silver nanoparticles using the extract of spent coffee used for paper-based hydrogen peroxide sensing device. Scientific Reports, 12(1), (2022) 20099. https://doi.org/10.1038/s41598-022-22067-6
M.A. Rashidi, S. Falahi, S. Farhang Dehghan, H. Ebrahimzadeh, H. Ghaneialvar, R. Zendehdel, Green synthesis of silver nanoparticles by Smyrnium cordifolium plant and its application for colorimetric detection of ammonia. Scientific Reports, 14(1), (2024) 24161. https://doi.org/10.1038/s41598-024-73010-w
Z.O. Uygun, S. Tasoglu, Impedimetric antimicrobial peptide biosensor for the detection of human immunodeficiency virus envelope protein gp120. Iscience, 27(3), (2024). https://doi.org/10.1016/j.isci.2024.109190
S.U. Pektaş, M. Keskin, O.C. Bodur, F. Arslan, Green synthesis of silver nanoparticles and designing a new amperometric biosensor to determine glucose levels. Journal of Food Composition and Analysis, 129, (2024)106133. https://doi.org/10.1016/j.jfca.2024.106133
T. Thongwattana, R. Chaiyo, K. Ponsanti, B. Tangnorawich, P. Pratumpong, S. Toommee, R. Jenjob, S.G. Yang, Y. Parcharoen, S. Natphopsuk, C. Pechyen, Synthesis of Silver Nanoparticles and Gold Nanoparticles Used as Biosensors for the Detection of Human Serum Albumin-Diagnosed Kidney Disease. Pharmaceuticals, 17(11), (2024) 1421. https://doi.org/10.3390/ph17111421
V. Rocha, Valorization of Plant By-Products in the Biosynthesis of Silver Nanoparticles. Immobilization of eco-friendly nanoparticles on different materials: towards promising nanoplatforms for removal of pollutants from water, (2024) 58. https://doi.org/10.1007/s11356-024-32180-w
Y. Jin, J. Lin, A. Sathiyaseelan, X. Zhang, M.H. Wang, Comparative studies on antibacterial, antibiofilm, antioxidant, and cytotoxicity properties of chemically and Paeonia lactiflora extract-assisted synthesized silver nitroprusside nanoparticles. Journal of Drug Delivery Science and Technology, 92, (2024) 105269. https://doi.org/10.1016/j.jddst.2023.105269
T.S. da Costa, M.R. da Silva, J.C.J. Barbosa, U.D.S.D. Neves, M.B. de Jesus, L. Tasic, Biogenic silver nanoparticles' antibacterial activity and cytotoxicity on human hepatocarcinoma cells (Huh-7). RSC advances, 14(4), (2024) 2192-2204. https://doi.org/10.1039/D3RA07733K
G.E. Okuthe, B. Siguba, Acute Dermatotoxicity of Green-Synthesized Silver Nanoparticles (AgNPs) in Zebrafish Epidermis. Toxics, 13(7), (2025) 592. https://doi.org/10.3390/toxics13070592
A. Rana, K. Yadav, S. Jagadevan, A comprehensive review on green synthesis of nature-inspired metal nanoparticles: Mechanism, application and toxicity. Journal of Cleaner Production, 272, (2020) 122880. https://doi.org/10.1016/j.jclepro.2020.122880
P. Nie, Y. Zhao, H. Xu, Synthesis, applications, toxicity and toxicity mechanisms of silver nanoparticles: A review. Ecotoxicology and Environmental Safety, 253, (2023) 114636. https://doi.org/10.1016/j.ecoenv.2023.114636
M. Madhusudanan, J. Zhang, S. Pandit, P. Singh, G.J. Jeong, F. Khan, I. Mijakovic, Green Synthesis of Silver Nanoparticles: A Review of Polymer and Antimicrobial Drug Combinations for Enhanced Antimicrobial Applications. Advanced NanoBiomed Research, (2025) 2400194. https://doi.org/10.1002/anbr.202400194
U. Ramzan, W. Majeed, A.A. Hussain, F. Qurashi, S.U.R. Qamar, M. Naeem, J. Uddin, A. Khan, A. Al-Harrasi, S.I.A. Razak, T.Y. Lee, New insights for exploring the risks of bioaccumulation, molecular mechanisms, and cellular toxicities of AgNPs in aquatic ecosystem. Water, 14(14), (2022) 2192. https://doi.org/10.3390/w14142192
A.M. Eid, S.E.D. Hassan, M.F. Hamza, S. Selim, M.S. Almuhayawi, M.H. Alruhaili, M.K. Tarabulsi, M.K. Nagshabandi, A. Fouda, Photocatalytic, antimicrobial, and cytotoxic efficacy of biogenic silver nanoparticles fabricated by Bacillus amyloliquefaciens. Catalysts, 14(7), (2024) 419. https://doi.org/10.3390/catal14070419
M. Muhamad, N. Ab. Rahim, W.A. Wan Omar, N.N.S. Nik Mohamed Kamal, Cytotoxicity and Genotoxicity of Biogenic Silver Nanoparticles in A549 and BEAS‐2B Cell Lines. Bioinorganic Chemistry and Applications, 2022(1), (2022) 8546079. https://doi.org/10.1155/2022/8546079
V.T. Veetil, V. Jayakrishnan, V. Aravindan, A.D. Rajeeve, S. Koolath, R. Yamuna, Biogenic silver nanoparticles incorporated hydrogel beads for anticancer and antibacterial activities. Scientific Reports, 14(1), (2024) 27269. https://doi.org/10.1038/s41598-024-79003-z
H. Singh, M.F. Desimone, S. Pandya, S. Jasani, N. George, M. Adnan, A. Aldarhami, A.S. Bazaid, S.A. Alderhami, Revisiting the green synthesis of nanoparticles: uncovering influences of plant extracts as reducing agents for enhanced synthesis efficiency and its biomedical applications. International journal of Nano medicine, (2023) 4727-4750. https://doi.org/10.2147/IJN.S419369
Y. Khane, K. Benouis, S. Albukhaty, G.M. Sulaiman, M.M. Abomughaid, A. Al Ali, D. Aouf, F. Fenniche, S. Khane, W. Chaibi, A. Henni, H.D. Bouras, N. Dizge, Green synthesis of silver nanoparticles using aqueous Citrus limon zest extract: Characterization and evaluation of their antioxidant and antimicrobial properties. Nanomaterials, 12(12), (2022) 2013. https://doi.org/10.3390/nano12122013
H. Duman, F. Eker, E. Akdaşçi, A.M. Witkowska, M. Bechelany, S. Karav, Silver Nanoparticles: A comprehensive review of synthesis methods and chemical and physical properties. Nanomaterials, 14(18), (2024) 1527. https://doi.org/10.3390/nano14181527
D.J.R. Rufina, H. Uthayakumar, P. Thangavelu, Prediction of the size of green synthesized silver nanoparticles using RSM-ANN-LM hybrid modeling approach. Chemical Physics Impact, 6, (2023) 100231. https://doi.org/10.1016/j.chphi.2023.100231
G.K. Gupta, D. Koli, R.K. Kapoor, Statistical optimization for greener synthesis of multi-efficient silver nanoparticles from the Hypocrea lixii GGRK4 culture filtrate and their ecofriendly applications. Frontiers in Nanotechnology, 6, (2024) 1384465. https://doi.org/10.3389/fnano.2024.1384465
J.M. Do, J.W. Hong, H.S. Yoon, Microalgae-mediated green synthesis of silver nanoparticles: a sustainable approach using extracellular polymeric substances from Graesiella emersonii KNUA204. Frontiers in Microbiology, 16, (2025) 1589285. https://doi.org/10.3389/fmicb.2025.1589285
F. Rodríguez-Félix, A.Z. Graciano-Verdugo, M.J. Moreno-Vásquez, I. Lagarda-Díaz, C.G. Barreras-Urbina, L. Armenta-Villegas, A. Olguín-Moreno, J.A. Tapia-Hernández, (2022). Trends in sustainable green synthesis of silver nanoparticles using Agri-Food waste extracts and their applications in health. Journal of Nanomaterials, 2022(1), (2022) 8874003. https://doi.org/10.1155/2022/8874003
S.I. Manzoor, F. Jabeen, R. Patel, M.M.A. Rizvi, K. Imtiyaz, M.A. Malik, T.A. Dar, Green synthesis of biocompatible silver nanoparticles using Trillium govanianum rhizome extract: comprehensive biological evaluation and in silico analysis. Materials Advances, 6(2), (2025) 682-702. https://doi.org/10.1039/D4MA00959B
M. Noga, J. Milan, A. Frydrych, K. Jurowski, Toxicological aspects, safety assessment, and green toxicology of silver nanoparticles (AgNPs)—critical review: state of the art. International Journal of Molecular Sciences, 24(6), (2023) 5133. https://doi.org/10.3390/ijms24065133
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